The Effect of Bacillus licheniformis on weight gain, Blood picture and Lipid Profiles in rats feed high Cholesterol diet

Abstract

  The study aims to detect the effect of adding of Bacillus licheniformis bacteria (1 × 108 cells/day/animal) to reduce the negative effect of cholesterol added to the food ration by 2% on body weights and the effect on some parameters, blood images and lipid profiles in male laboratory rats after a period of 28 days. In this study, 15 adult male Albino Sprague- Dawleyweanling rats of 8-9 weeks of age and average weight of 142 g are used. The animals are randomly distributed into 3 groups with each group consisting of 5 animals. The results show that the addition of cholesterol causes a significant increase at (P<0.05) in body weight, as the weight gain is 43.18 g compared with 19.13 g in the control group. The results also show a decrease in total number of red blood cells, hemoglobin levels, HCT and a significant increase in the number of white blood cells and platelets for groups of rats feeding cholesterol at (6.12 , 10.44 , 35.32 , 11.94 , 850.60) compared with the control group (7.09 , 12.30 , 38.54 , 9.22 , 597.40) respectively. The results also show an increase in the level of cholesterol, LDL and triglycerides, and a significant decrease in the level of HDL compared to the control group.

     The addition of Bacillus licheniformis with cholesterol has a significant effect in reducing the negative effect of cholesterol on all the above-measured parameters.